Microprocessor UV-VIS Double Beam Spectrophotometer

Popularity of Double Beam Spectrophotometer & Uv-Vis Spectrophotometer is getting increased spectacularly.

It has a range from 190-1100 nm, basic/quantities wavelength scan/DNA Protein Test/Kinetic, automatic peak picking & spectrum display. It has a large screen with graphic display. Spectrum and data can be printed out by printer and sent to computer via USB PORT. Set of 4 quartz and 4 glass cuvettes is provided. SOFTWARE INCLUDED

This is advanced Double Beam UV-VIS Spectrophotometer with more accuracy and flexible requirement. The two detectors are used to measure sample and reference respectively and simultaneously for optimizing measurement accuracy. It has vide wavelength range satisfying requirement of various fields, such as biochemical research and industry, pharmaceuticals analysis and production, education, environment protection, food industry etc.

Basic Mode

To measure the absorbance and transmittance


1. Coefficient Method

2. Standard Curve

Up to 10 standard samples may be used to establish a curve, four methods for fitting a curve through the calibration points: Linear fit, linear fit through zero. Square fit and cubic fit.

DNA/Protein Test

Concentration and DNA purity are quickly and easily calculated: Absorbance ratios 260 nm / 280 nm with optional subtracted absorbance at 320 nm. DNA concentration=62.9 x A260+36.0x A280 protein concentration+552xA260-757xA280

Wavelength Scan

1. The wavelength scan intervals are 0.1, 0.2, 0.5,1,2,5 nm

2. Hi, Medium and Low scan speeds are available. They vary from 100 to 3600 nm/Min.

3. Wavelengths are scanned from high to low so that the instrument waits at high WL. And it minimize the degradation of UV sensitive samples.


This mode may be used for time course scanning or reaction rate calculations. Abs vs time graphs is displayed on the screen on real time.

Wait time and measurement time up to 12 hours may be entered with time intervals of 0.5,1,2,5,10,30 seconds & 1 min. Past-run manipulation includes using a linear regression algorithm before multiplying by the entered factor

Glass Cells4 No.
Quartz Cells4 No.
Fuse2 No.
Instrument Cover1 No.
Software CD1 No.
USB Cable 1 No.
Operational Manual 1 No.

Optical SystemDouble Beam (1200 Lines / mm Grating)
Wavelength Range190 – 1100 nm
ModeBasic / Quantitative / Wavelength Scan / DNA Protein Test / Kinetics
Scanning SpeedFast / Medium / Low
Band Width1 nm
Wavelength Accuracy+/- 0.1 nm
Wavelength0.1 nm
Photometric Accuracy+/- 0.3 % T
Photometric Repeatability0.2 % T
Photometric Display Range0-200%T, 0.3-3.0A, 0.9999C
Stability0.001A/h @ 500 nm
Baseline Flatness+/- 0.001A
Noise+/- 0.001A
Stray Light< 0.005%T@ 220nm, 360nm
Data Output PortUSB
Printer PortParallel Port
Display Graphic LCD (320 x 240 Dots)
LampsDeuterium Lamp & Tungsten Halogen Lamp
Detector Silicon Photodiodes
Dimension (L x W x H)625 x 430 x 206 nm
Net Weight26 Kg